MAP Kinase Pathways in Brain Endothelial Cells and Crosstalk with Pericytes and Astrocytes Mediate Contrast-Induced Blood–Brain Barrier Disruption

Neurointervention with contrast media (CM) has rapidly increased, but the impact of CM extravasation and related side effects remain controversial. This study investigated effect on blood–brain barrier (BBB) integrity. We established in vitro BBB models using primary cultures rat BBB-related cells. To assess functions, we evaluated transendothelial electrical resistance, permeability, tight junction (TJ) protein expression immunohistochemistry (IHC) Western blotting. investigate mechanism iopamidol-induced dysfunction, role mitogen-activated (MAP) kinases brain endothelial cells was examined. assessed conditioned medium derived from astrocytes pericytes under iopamidol treatment. Short-term exposure luminal induced transient, while abluminal caused persistent dysfunction. IHC immunoblotting revealed decreased TJ proteins. Iopamidol-induced dysfunction improved via regulation MAP kinase pathways. Conditioned CM-exposed or lacks ability to enhance function. may cause pathways interactions mediate have negative clinical outcomes patients.